A simple method for the isolation of islets of Langerhans from rat pancreas
نویسندگان
چکیده
Rat hearts were perfused for 15 min with the concentration of glucose indicated, with or without insulin (1 munit/ml). The tissue was freeze-clamped. Fructose 2,6-bisphosphate content and PFK-2 activity in 20% (w/v) polyethylene glycol fraction were measured as described by Rider & Hue (1984). Values are means ~ s . E . M . for the number of experiments shown in parentheses. LDH, Lactate dehydrogenase.
منابع مشابه
Protective Effect of Capparis spinosa Hydroalcoholic Extract on the Integrity of Rat Pancreatic Islets
Background and Objective: Capparis spinosa L. belongs to the Capparaceae family for which biological roles such as antioxidant, anti-inflammatory, and antidiabetic effects have been reported. Some active chemical groups including flavonoids, phenols, alkaloids, tannins, and minerals have been detected in these plants [Sci Rew1] . This study aimed to extract the C. spinosa alcoholic extract from...
متن کاملMONOPHASIC STATE OF INSULIN SECRETION IN ISOLATED RAT ISLETS OF LANGERHANS BY K+, Ca2+ AND GLUCOSE
Isolation of rat islets of Langerhans was carried out by using collagenase and the destruction of exocrine tissue was performed through the injection of Hanks' solution into the common bile duct. The identification of the islets was done by techniques including the use of a stereomicroscope against a black background. Vital staining was done by an injection of neutral red solution through t...
متن کاملEffect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets
Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containi...
متن کاملHistomorphometrical study of pancreas in Mehraban female sheep
In the present study, pancreas glands in Mehraban breed of female sheep were histomorphometricallystudied in four different age groups included: fetus, newborn, sexually mature and old ages. The results ofhistomorphometrical analysis with regard to frequency and percentage of parenchymal and stromal structures such as, exocrine secretory units, secretory ducts, blood vessels, islets of Langerha...
متن کاملاثر محافظتی آلوئهورا بر روی ساختار بافتی بخش درونریز پانکراس موش صحرایی دیابتی
Introduction: Since aloe vera plant has many medical benefits, the present study aimed to investigate the protective effects of Aloe vera gel on the pancreatic islets and beta cells. Methods: This experimental study consisted of 50 mature male rats aged 2-3 months and weighed 200-250 g, who were randomly divided into five groups (n=10). Group I (control) did not receive any treatments, and g...
متن کاملThe effect of ghrelin on Kiss-1 and KissR gene transcription and insulin secretion in rat islets of Langerhans and CRI-D2 cell line
Objective(s): Ghrelin is a peptide hormone that has been shown to have numerous central and peripheral effects. The central effects including GH secretion, food intake, and energy homeostasis are partly mediated by Kiss1- KissR signaling pathway. Ghrelin and its receptor are also expressed in the pancreatic islets. Ghrelin is one of the key metabolic factors controlling insulin secretion from t...
متن کامل